Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade,Abcam,AB213224

Product Specifications Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) is a rabbit recombinant monoclonal antibody and is validated for use in CUT&Tag, ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Dot Blot, Flow Cyt (Intra), ICC/IF, IP, PepArr, WB in human, mouse and rat samples. Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) specifically detects Histone H3 Tri Methyl-K4 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 µL and 1 mL selling sizes. Quality and Validation Abcam's high quality manufacturing and validation processes ensure Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) has been cited over 22 times in peer reviewed journals and is trusted by the scientific community. Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) has 5 independent reviews from customers. Related Products Conjugation-ready, carrier free format available for antibody clone EPR20551-225 - ab313500 . Antibody clone EPR20551-225 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, PE ( ab237340 , ab237341 , ab237342 ). Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

Dot, ICC/IF, IP, ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Flow Cyt (Intra), WB, PepArr, CUT&Tag

Platform ID

BAB020578147

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade
Cat. No.AB213224
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationDot, ICC/IF, IP, ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Flow Cyt (Intra), WB, PepArr, CUT&Tag
ClonalityMonoclonal
Clone NumberEPR20551-225
Concentration0.49 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. See full target information H3C1 tri methyl K4

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