Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade,Abcam,AB176916

Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in CUT&Tag, ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Dot, ICC/IF, IHC-P, PepArr, WB in human, mouse, rat samples. Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) has been cited over 54 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality manufacturing and validation processes ensure Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) has 9 independent reviews from customers. Anti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade (ab176916) specifically detects Histone H3 Tri Methyl-K9 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 uL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR16601 - ab232324 . Antibody clone EPR16601 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, PE, APC, Alexa Fluor® 75 ( ab284635 , ab288339 , ab32226, ab32227 , ab32228, ab32229, ab32119 ). Histone H3 (tri methyl K9), also known as H3K9me3, is an important epigenetic modification associated with heterochromatin formation and gene silencing. This antibody is crucial in cancer research, particularly in understanding H3K9me3's role in gene silencing and heterochromatin formation, and is widely used in studies of transcriptional repression and histone modifications involving histone methyltransferases. Key histone marker that has been implicated in both transcriptional activation and silencing. The tri methyl K9 antibody is essential for studying this modification's role in transcription regulation, DNA repair, and chromosomal stability. Abnormal H3K9me3 levels are linked to various diseases, including cancer and neurological disorders. Monitoring H3K9me3 can provide valuable insights into disease mechanisms and potential therapeutic targets. Validated for CUT&Tag, CUT&RUN and ChIP-seq, which are key applications to map protein-DNA interactions on a genome-wide scale using NGS. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

WB, ICC/IF, Dot, ChIP-seq, ChIC/CUT&RUN-seq, IHC-P, PepArr, CUT&Tag, ChIP

Platform ID

BAB083159358

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Histone H3 (tri methyl K9) antibody [EPR16601] - ChIP Grade
Cat. No.AB176916
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationWB, ICC/IF, Dot, ChIP-seq, ChIC/CUT&RUN-seq, IHC-P, PepArr, CUT&Tag, ChIP
ClonalityMonoclonal
Clone NumberEPR16601
Concentration0.6795 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically. See full target information H3C1 tri methyl K9

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