Anti-Human CD32 (FcγRIIA) [Clone IV.3] — Purifiedin vivoGOLD™ Functional Grade,Leinco,C1284

Host

Mouse

Reactivity

Human

Application

ELISA,FC,WB

Platform ID

BAB696873139

Leinco

Headquarters

410 Axminister Drive St. Louis, Missouri 63026

Leinco

Contact

Tel: +1 (800) 538-1145,+1 (636) 230-9477
Fax:

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-Human CD32 (FcγRIIA) [Clone IV.3] — Purifiedin vivoGOLD™ Functional Grade
Cat. No.C1284
HostMouse
ReactivityHuman
ApplicationELISA,FC,WB
Clone NumberIV.3
Concentration≥ 5.0 mg/ml
ImmunogenHuman K562 leukemia cell line
Purity≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Endotoxin Level< 1.0 EU/mg as determined by the LAL method
Shipping2-8°C wet ice
FormulationThis monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
StorageFunctional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Scientific Background

The Anti-Human CD32 monoclonal antibody (Clone IV.3) specifically reacts with human CD32, a 40 kDa transmembrane glycoprotein expressed on monocytes, macrophages, B cells, and platelets. As an Fc receptor (FcγRIIA), CD32 mediates vital immune responses such as phagocytosis, ADCC, degranulation, and cytokine release by interacting with IgG-immune complexes. Clone IV.3 binds directly to the ligand-binding site, making it a powerful tool for blocking the functional effects of CD32 in vitro. Additionally, it is highly utilized in flow cytometry to eliminate non-specific background staining by effectively blocking Fc-receptors prior to antibody labeling.

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