Anti-Human CD8a [Clone OKT-8] — Purifiedin vivoGOLD™ Functional Grade,Leinco,C1071

CD8 is an integral membrane protein essential to immune response that acts as a co-receptor for the MHC class I molecule:peptide complex 1 . CD8 recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex, leading to the activation of cytotoxic T-lymphocytes which recognize and eliminate infected cells and tumor cells. CD8 + T cell differentiation is tightly regulated, and the T cell response depends on the antigen encounter. Immune response to acute infection, autoimmunity, graft vs host disease, tumors, chronic infection, and self-tolerance are all affected by CD8 + T cells 2 . CD8 molecules are either CD8α/CD8β heterodimers, when expressed on thymus-derived T cells, or they are CD8α homodimers, when expressed on intraepithelial lymphocytes from the gut, CD8 + dendritic cells 3 , or a subset of human natural killer cells 4 . Gene expression is closely regulated with CD4, with expression of CD4 and CD8 used to distinguish the four major stages of T cell development 4 . OKT8 was generated by immunizing a CAF 1 /J mouse with human T lymphocytes 5,6 . Spleen cells were fused with P3X63Ag8.U1 myeloma cells and OKT8 was shown to be highly specific for human T cell populations by indirect immunofluorescence and C-mediated microcytotoxic assays. OKT8 can fix complement 7 .