Anti-INCENP antibody,Abcam,AB12183

If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. There is some debate about whether this antibody is suitable for use in IF and we would be most interested to hear from researchers who test this antibody in this application. The antibody has been reported to be suitable for use in IF at a dilution of 1/100 using HeLa cells (see image below). However, one of our customers used this product in HeLa cells and reported an unusual staining pattern - please see the enquiries section of the online datasheet for further details.

Host

Rabbit

Reactivity

Mouse, Rat, Human, Xenopus laevis

Application

ICC/IF, IHC-Fr, ICC, WB, IP

Platform ID

BAB042667539

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-INCENP antibody
Cat. No.AB12183
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human, Xenopus laevis
ApplicationICC/IF, IHC-Fr, ICC, WB, IP
ClonalityPolyclonal
Concentration12.3 mg/mL Batch dependent concentration
ImmunogenSynthetic Peptide within Human INCENP aa 850 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules (PubMed : 12925766, PubMed : 15316025, PubMed : 27332895). The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity). Activates AURKB and AURKC (PubMed : 27332895). Required for localization of CBX5 to mitotic centromeres (PubMed : 21346195). Controls the kinetochore localization of BUB1 (PubMed : 16760428). See full target information INCENP

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.