Anti-INO80 antibody [EPR30384-761] - BSA and Azide free,Abcam,AB325543

ab325543 is the carrier-free version of ab325542 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.

Host

Rabbit

Reactivity

Human, Mouse

Application

ChIC/CUT&RUN-seq, ICC/IF, ChIP-seq, WB

Platform ID

BAB918504637

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-INO80 antibody [EPR30384-761] - BSA and Azide free
Cat. No.AB325543
HostRabbit
IsotypeIgG
ReactivityHuman, Mouse
ApplicationChIC/CUT&RUN-seq, ICC/IF, ChIP-seq, WB
ClonalityMonoclonal
Clone NumberEPR30384-761
Concentration1.022 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data ATPase component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and DNA repair (PubMed : 16230350, PubMed : 16298340, PubMed : 17721549, PubMed : 20237820, PubMed : 20855601). Binds DNA (PubMed : 16298340, PubMed : 21303910). As part of the INO80 complex, remodels chromatin by shifting nucleosomes (PubMed : 16230350, PubMed : 21303910). Regulates transcription upon recruitment by YY1 to YY1-activated genes, where it acts as an essential coactivator (PubMed : 17721549). Involved in UV-damage excision DNA repair (PubMed : 20855601). The contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation (PubMed : 20687897). Involved in DNA replication (PubMed : 20237820). Required for microtubule assembly during mitosis thereby regulating chromosome segregation cycle (PubMed : 20237820). See full target information INO80

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