Anti-IRF5 antibody [10T1],Abcam,AB33478

What is this antibody validated in? Anti-IRF5 antibody [10T1] (ab33478) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples. What is the molecular weight of IRF5? Anti-IRF5 [10T1] (ab33478) specifically detects a band for IRF5 (UniProt: Q13568) at a molecular weight of 58kDa. Trusted by the scientific community Anti-IRF5 [10T1] (ab33478) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals. Specificity confirmed The specificity of Anti-IRF5 antibody [10T1] (ab33478) has been confirmed by Western blot testing in IRF5 Knockout HAP1 cells.

Host

Mouse

Reactivity

Mouse, Human

Application

ICC/IF, WB, Flow Cyt (Intra)

Platform ID

BAB172227849

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-IRF5 antibody [10T1]
Cat. No.AB33478
HostMouse
IsotypeIgG1
ReactivityMouse, Human
ApplicationICC/IF, WB, Flow Cyt (Intra)
ClonalityMonoclonal
Clone Number10T1
Concentration1 mg/mL Batch dependent concentration
ImmunogenRecombinant Fragment Protein within Human IRF5. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein G
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed : 11303025, PubMed : 15695821, PubMed : 22412986, PubMed : 25326418, PubMed : 32433612). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (By similarity). Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway (By similarity). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148). See full target information IRF5

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