Anti-Iba1 antibody [EPR16589] - BSA and Azide free,Abcam,AB221790

What is this antibody validated in? Anti-Iba1 antibody [EPR16589] - BSA and Azide free (ab221790) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of Iba1? Anti-Iba1 [EPR16589] - BSA and Azide free (ab221790) specifically detects a band for Iba1 (UniProt: P55008) at a molecular weight of 17kDa. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [EPR16589] also available for your convenience: ab178847 , HRP - ab216544 , Carrier free - ab221790, ab283319 , Carrier free - ab283342 , ab283346 , Alexa Fluor® 488 - ab310987 , Alexa Fluor® 647 - ab311112 , Alexa Fluor® 594 - ab311734 , Alexa Fluor® 488 - ab312913 , Alexa Fluor® 568 - ab313011 , Alexa Fluor® 555 - ab313215 , Alexa Fluor® 750 - ab321219 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

IHC (PFA fixed), IP, IHC-Fr, WB, ICC/IF, IHC-P

Platform ID

BAB033771811

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Iba1 antibody [EPR16589] - BSA and Azide free
Cat. No.AB221790
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationIHC (PFA fixed), IP, IHC-Fr, WB, ICC/IF, IHC-P
ClonalityMonoclonal
Clone NumberEPR16589
Concentration1.061 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. See full target information AIF1

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