Anti-Iba1 antibody [EPR16589] - Microglia marker - Rat IgG2a (Chimeric),Abcam,AB283346

Find all reagents to label astrocytes in our " Microglia markers guide ". Product Specifications Anti-Iba1 antibody [EPR16589] - Rat IgG2a (Chimeric) (ab283346) is a rat monoclonal recombinant antibody validated for use in IHC-P, IP, WB, ICC/IF and Flow Cyt (Intra) in mouse, rat and human samples. Clone EPR16589 was initially developed by Abcam using patented rabbit monoclonal antibody technology and has been engineered to generate this rat chimeric antibody which retains the binding properties of the RabMAb parent, but has a rat Fc region. By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended. Anti-Iba1 antibody [EPR16589] - Rat IgG2a (Chimeric) (ab283346) specifically detects Iba1 (AIF-1) (UniProt ID: P55008; Molecular weight: 17kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Quality and Validation Abcams high quality manufacturing and validation processes ensure Anti-Iba1 antibody [EPR16589] - Rat IgG2a (Chimeric) (ab283346) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Target Information Iba1 (Ionized calcium-binding adapter molecule 1; allograft inflammatory factor 1, AIF1) is a prominent microglial marker extensively used in neuroscience research. Its expression increases in various neurodegenerative conditions, including Alzheimer's disease (AD), indicating microglial activation and neuroinflammation. Researchers utilize Iba1 in stainings to assess microglial response and activity in different models, making it essential for understanding inflammatory processes in the brain. Changes in Iba1 expression provide valuable insights into neuroinflammation, highlighting its importance in studying diseases like AD. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .