Anti-KLF4 antibody [EPR22833-30] - BSA and Azide free (Detector),Abcam,AB281107
ab281107 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse KLF4. The recommended pair for sandwich ELISA is: Capture: ab281257 , Mouse KLF4 Capture Antibody (unconjugated) Detector: ab281107, Mouse KLF4 Detector Antibody (unconjugated) The reference range value is 0.15 - 10 ng/ml. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Mouse, Rat
Application
sELISA
Platform ID
BAB240906132
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence (PubMed : 10431239, PubMed : 10556311, PubMed : 15358627, PubMed : 16954384, PubMed : 17060454, PubMed : 19816951, PubMed : 20071344, PubMed : 29593216). Binds to the promoter region of its own gene and can activate its own transcription (PubMed : 10431239, PubMed : 10556311, PubMed : 15358627, PubMed : 16954384, PubMed : 17060454, PubMed : 19816951, PubMed : 20071344, PubMed : 29593216). Regulates the expression of key transcription factors during embryonic development (PubMed : 10431239, PubMed : 10556311, PubMed : 15358627, PubMed : 16954384, PubMed : 17060454, PubMed : 19816951, PubMed : 20071344, PubMed : 29593216). Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation (PubMed : 10431239, PubMed : 10556311, PubMed : 15358627, PubMed : 16954384, PubMed : 17060454, PubMed : 19816951, PubMed : 20071344, PubMed : 29593216). Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity). See full target information Klf4
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