Anti-Ki67 antibody [EPR20582-7] - BSA and Azide free (Detector),Abcam,AB252656
What is this antibody validated in? Anti-Ki67 antibody [EPR20582-7] - BSA and Azide free (Detector) (ab252656) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA in Human samples. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Human
Application
sELISA
Platform ID
BAB552013051
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data The protein expressed by the MKI67 gene is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly and associates with the surface of the mitotic chromosome, specifically the perichromosomal layer, covering a significant portion of the chromosome surface (PubMed : 27362226). It prevents chromosomes from collapsing into a single chromatin mass by creating a steric and electrostatic charge barrier due to its high net electrical charge, acting as a surfactant that disperses chromosomes and enables their independent motility (PubMed : 27362226). The protein binds DNA, with a preference for supercoiled and AT-rich DNA (PubMed : 10878551), and may play a role in chromatin organization, though it is unclear if it directly influences chromatin organization or if this is an indirect result of its function in maintaining dispersed mitotic chromosomes (Probable, PubMed : 24867636). This supplementary information is collated from multiple sources and compiled automatically. See full target information MKI67
Category Paths
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