Anti-Ki67 antibody [SP6] - BSA free,Abcam,AB231172
What is this antibody validated in? Anti-Ki67 antibody [SP6] - BSA free (ab231172) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human, Mouse, Rat samples. What is the molecular weight of Ki67? Anti-Ki67 [SP6] - BSA free (ab231172) specifically detects a band for Ki67 (UniProt: P46013) at a molecular weight of 358kDa. Collaboration Anti-Ki67 [SP6] - BSA free (ab231172) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC). Trusted by the scientific community Anti-Ki67 [SP6] - BSA free (ab231172) was first used in a scientific publication in 2019 and has been cited over 20 times in peer-reviewed journals. Specificity confirmed The specificity of Anti-Ki67 antibody [SP6] - BSA free (ab231172) has been confirmed by Flow Cytometry (Intracellular) testing in MKI67 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [SP6] also available for your convenience: ab16667 , ab21700 , Carrier free - ab197547 , ab231172, ab238624, Oligonucleotide - ab279361, Alexa Fluor® 488 - ab281847 , Alexa Fluor® 647 - ab281928 , PE - ab282173 , APC - ab314285 , ab320709 , Carrier free - ab320710 , ab323292 What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
Flow Cyt (Intra), ICC/IF, WB, mIHC, IHC-P
Platform ID
BAB364569274
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis (PubMed : 27362226, PubMed : 32879492, PubMed : 35513709, PubMed : 39153474). Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed : 27362226). During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface (PubMed : 27362226). The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier : the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed : 27362226). During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space (PubMed : 32879492, PubMed : 39153474). Mechanistically, dephosphorylation during mitotic exit and simultaneous exposure of a conserved basic patch induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface, promoting coalescence of neighboring chromosome surfaces and clustering of chromosomes (PubMed : 39153474). Binds premature ribosomal RNAs during anaphase; promoting liquid-liquid phase separation (PubMed : 28935370, PubMed : 39153474). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed : 10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization; it is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in mitotic chromosome (PubMed : 24867636). See full target information MKI67
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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