Anti-Ku70 antibody [EPR4027] - BSA and Azide free,Abcam,AB239912
ab239912 is the carrier-free version of ab92450 . Species reactivity Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.
Host
Rabbit
Reactivity
Human
Application
WB, Flow Cyt (Intra), ICC/IF, IHC-P, IP
Platform ID
BAB117043086
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). Required for double-strand break repair and V(D)J recombination (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). Also has a role in chromosome translocation (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). Has a role in chromosome translocation (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). It works in the 3'-5' direction (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step : it recognizes and binds to the broken ends of the DNA and protects them from further resection (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). Binding to DNA may be mediated by XRCC6 (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step (PubMed : 11493912, PubMed : 12145306, PubMed : 20493174, PubMed : 2466842, PubMed : 7957065, PubMed : 8621488, PubMed : 9742108). Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks (PubMed : 20383123). 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined (PubMed : 20383123). The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed : 8621488). In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed : 12145306). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed : 28712728). See full target information XRCC6
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- Products>Primary Antibodies>IHC Antibodies
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