Anti-LEF1 antibody [EPR2029Y],Abcam,AB137872
Anti-LEF1 antibody [EPR2029Y] (ab137872) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB. Abcam's high quality manufacturing and validation processes ensure Anti-LEF1 antibody [EPR2029Y] (ab137872) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-LEF1 antibody [EPR2029Y] (ab137872) has been confirmed by Western Blot testing in LEF1 knockout Jurkat cells ( ab274898 ). Anti-LEF1 antibody [EPR2029Y] (ab137872) specifically detects LEF1 (UniProt ID: Q9UJU2; Molecular weight: 45kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EPR2029Y - ab215999 . Antibody clone EPR2029Y is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor ® 647, APC ( ab197623 , ab246715 , ab314938 ). LEF1 is a transcription factor that promotes tumorigenicity in various cancers by activating pathways like TGF-β. LEF1 also plays a role in maintaining cancer stem cell properties, contributing to chemoresistance and metastasis. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
ICC/IF, IHC-P, Flow Cyt (Intra), IP, WB
Platform ID
BAB745553900
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Transcription factor that binds DNA in a sequence-specific manner (PubMed : 2010090). Participates in the Wnt signaling pathway (By similarity). Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (PubMed : 11266540). Regulates T-cell receptor alpha enhancer function (PubMed : 19653274). Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity). May play a role in hair cell differentiation and follicle morphogenesis (By similarity).. Isoform 1. Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.. Isoform 3. Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling.. Isoform 5. Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. See full target information LEF1_HUMAN
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
- Products>Trial Size Antibodies
Please provide the required information below so that we can quickly source your products.