Anti-LIM Kinase 1 antibody,Abcam,AB217690

Target data Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways (PubMed : 10436159, PubMed : 11832213, PubMed : 12807904, PubMed : 15660133, PubMed : 16230460, PubMed : 18028908, PubMed : 22328514, PubMed : 23633677). Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop (PubMed : 10436159). LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton (PubMed : 11832213, PubMed : 15660133, PubMed : 16230460, PubMed : 23633677). In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation (PubMed : 11832213, PubMed : 15660133, PubMed : 16230460, PubMed : 23633677). Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly (PubMed : 18028908). Stimulates axonal outgrowth and may be involved in brain development (PubMed : 18028908).. Isoform 3. Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1). See full target information LIMK1