Anti-Legumain antibody [EPR18150-36] - BSA and Azide free (Detector),Abcam,AB242801
ab242801 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Legumain. The recommended pair for sandwich ELISA is: Capture: ab242553 , Mouse Legumain Capture Antibody (unconjugated) Detector: ab242801, Mouse Legumain Detector Antibody (unconjugated) The reference range value is 125 - 8000 pg/ml. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Mouse
Application
sELISA
Platform ID
BAB745653852
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Has a strict specificity for hydrolysis of asparaginyl bonds (PubMed : 24407422, PubMed : 9742219, PubMed : 9891971). Can also cleave aspartyl bonds slowly, especially under acidic conditions (PubMed : 24407422, PubMed : 9742219, PubMed : 9891971). Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (By similarity). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol (PubMed : 37347855). Required for normal lysosomal protein degradation in renal proximal tubules (PubMed : 17350006, PubMed : 21292981). Required for normal degradation of internalized EGFR (PubMed : 21292981). Plays a role in the regulation of cell proliferation via its role in EGFR degradation (PubMed : 21292981). See full target information Lgmn
Category Paths
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