Anti-MLH1 antibody [EPR3894],Abcam,AB92312
What is this antibody validated in? Anti-MLH1 antibody [EPR3894] (ab92312) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of MLH1? Anti-MLH1 [EPR3894] (ab92312) specifically detects a band for MLH1 (UniProt: P40692) at a molecular weight of 84kDa. Trusted by the scientific community Anti-MLH1 [EPR3894] (ab92312) was first used in a scientific publication in 2010 and has been cited over 80 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-MLH1 antibody [EPR3894] (ab92312) has been confirmed by Western blot testing in MLH1 Knockout HAP1 cell line, ab276105 . Other related products We have a range of other formats of antibody clone [EPR3894] also available for your convenience: ab92312, Alexa Fluor® 488 - ab199237 , Alexa Fluor® 647 - ab199494 , Carrier free - ab214441 , Alexa Fluor® 555 - ab215303 , PE - ab303047 , APC - ab303048 , HRP - ab303049 , Alkaline Phosphatase - ab308849 , Alexa Fluor® 594 - ab310577 , Alexa Fluor® 568 - ab312589 , Alexa Fluor® 750 - ab321745 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Shipping conditions update: ambient shipping This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality. Why the change? It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene. What you need to know Ambient shipments come clearly marked on the delivery note. No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products. Warranty coverage remains fully valid, aligned with our validated shipping method. Please store the product as per the datasheet instructions upon receipt. Find out more - https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
IHC-P, WB, ICC/IF, Flow Cyt (Intra)
Platform ID
BAB199014985
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis. See full target information MLH1
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
- Products>Trial Size Antibodies
Please provide the required information below so that we can quickly source your products.