Anti-MRas antibody [EPR12457],Abcam,AB176570

What is this antibody validated in? Anti-MRas antibody [EPR12457] (ab176570) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse samples. What is the molecular weight of MRas? Anti-MRas [EPR12457] (ab176570) specifically detects a band for MRas (UniProt: O14807) at a molecular weight of 24kDa. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-MRas antibody [EPR12457] (ab176570) has been confirmed by Western blot testing in MRAS Knockout HeLa cell line, ab265510 . Other related products We have a range of other formats of antibody clone [EPR12457] also available for your convenience: ab176570, Carrier free - ab240194 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Human

Application

IP, WB

Platform ID

BAB701363857

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-MRas antibody [EPR12457]
Cat. No.AB176570
HostRabbit
IsotypeIgG
ReactivityMouse, Human
ApplicationIP, WB
ClonalityMonoclonal
Clone NumberEPR12457
Concentration0.16 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival (PubMed : 16630891, PubMed : 28289718, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed : 16630891, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). The formation of the SMP complex only occurs when MRAS is GTP-bound (PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed : 16630891, PubMed : 35768504, PubMed : 35830882, PubMed : 35831509, PubMed : 36175670). See full target information MRAS

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