Anti-MTCO1 antibody [1D6E1A8],Abcam,AB14705
Anti-MTCO1 antibody [1D6E1A8] (ab14705) is a House Mouse recombinant monoclonal antibody and is validated for use in Flow Cyt, ICC, IHC-P, WB in human, recombinant fragment samples. Anti-MTCO1 antibody [1D6E1A8] (ab14705) has been cited over 521 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality manufacturing and validation processes ensure Anti-MTCO1 antibody [1D6E1A8] (ab14705) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-MTCO1 antibody [1D6E1A8] (ab14705) has 40 independent reviews from customers. Anti-MTCO1 antibody [1D6E1A8] (ab14705) specifically detects MTCO1 (UniProt ID: P00395; Molecular weight: 57kDa) and is sold in 100 ug and 1 mg selling sizes. Antibody clone 1D6E1A8 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 ( ab154477 , ab1986 ). This product has switched from a hybridoma to recombinant production method on 24th May 2023. Western blot protocol advice: For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal. Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio. This antibody clone [1D6E1A8] is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact [email protected]. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Mouse
Reactivity
Mouse, Rat, Human
Application
Flow Cyt, ICC, WB, IHC-P
Platform ID
BAB890606770
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. See full target information MT-CO1
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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