Anti-MeCP2 antibody [EPR23201-3],Abcam,AB253197

What is this antibody validated in? Anti-MeCP2 antibody [EPR23201-3] (ab253197) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of MeCP2? Anti-MeCP2 [EPR23201-3] (ab253197) specifically detects a band for MeCP2 (UniProt: P51608) at a molecular weight of 52kDa. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-MeCP2 antibody [EPR23201-3] (ab253197) has been confirmed by Western blot testing in MECP2 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR23201-3] also available for your convenience: ab253197, Carrier free - ab273059 , Carrier free - ab324009 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

IHC-P, WB, Flow Cyt (Intra), IP, ICC/IF

Platform ID

BAB194217289

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-MeCP2 antibody [EPR23201-3]
Cat. No.AB253197
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationIHC-P, WB, Flow Cyt (Intra), IP, ICC/IF
ClonalityMonoclonal
Clone NumberEPR23201-3
Concentration0.64 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). See full target information MECP2

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