Anti-MiTF antibody [C5],Abcam,AB12039
What is this antibody validated in? Anti-MiTF antibody [C5] (ab12039) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples. What is the molecular weight of MiTF? Anti-MiTF [C5] (ab12039) specifically detects a band for MiTF (UniProt: O75030) at a molecular weight of 59kDa. Trusted by the scientific community Anti-MiTF [C5] (ab12039) was first used in a scientific publication in 2004 and has been cited over 110 times in peer-reviewed journals. Reviewed by scientists Anti-MiTF [C5] (ab12039) has over 5 independent reviews from customers. Other related products We have a range of other formats of antibody clone [C5] also available for your convenience: ab12039, Carrier free - ab264110 Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected]
Host
Mouse
Reactivity
Human
Application
Flow Cyt (Intra), WB, ICC/IF
Platform ID
BAB448340309
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Involved in the cellular response to amino acid availability by acting downstream of MTOR : in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation (PubMed : 36608670). Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity (PubMed : 36608670). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). See full target information MITF
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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