Anti-Myc tag antibody [9E10],Abcam,AB32

Anti-Myc tag antibody [9E10] (ab32) is a House Mouse Monoclonal antibody and is validated for use in ELISA, Flow Cyt, ICC/IF, IHC-Fr, IP, Purification, WB in guinea pig, human, mouse, rat samples. Anti-Myc tag antibody [9E10] (ab32) has been cited over 432 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality validation processes ensure Anti-Myc tag antibody [9E10] (ab32) has high sensitivity and specificity. Anti-Myc tag antibody [9E10] (ab32) has 29 independent reviews from customers. Myc tag antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-Myc tag antibody [9E10] (ab32) has been validated in ELISA, Flow Cyt, ICC/IF, IHC-Fr, IP, Purification, WB Anti-Myc tag antibody [9E10] (ab32) specifically detects Myc tag (UniProt ID: P01106; Molecular weight: 51kDa) and is sold in 100 ug and 1 mg selling sizes. Conjugation-ready, carrier free format available for antibody clone 9E10 - ab26463. The Myc tag is a small peptide tag derived from the c-Myc protein, commonly used in molecular biology and biochemistry. It consists of 10 amino acids with the sequence EQKLISEEDL. This tag is often fused to proteins of interest to facilitate their detection and purification. Myc-tagged proteins can be identified using specific antibodies in various assays, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (ICC, IF), ELISA, and flow cytometry (FC). The Myc tag is a valuable tool for studying protein expression, localization, and interactions in diverse biological research applications. If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact [email protected] or you can find further information here .

Host

Mouse

Application

IHC-Fr, ELISA, IP, WB, ICC/IF, Purification, Flow Cyt

Platform ID

BAB142830702

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Myc tag antibody [9E10]
Cat. No.AB32
HostMouse
IsotypeIgG1
ApplicationIHC-Fr, ELISA, IP, WB, ICC/IF, Purification, Flow Cyt
ClonalityMonoclonal
Clone Number9E10
Concentration1 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808). See full target information MYC

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