Anti-Myc tag antibody [9E10],Abcam,AB206486

What is this antibody validated in? Anti-Myc tag antibody [9E10] (ab206486) is a rat recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF). Tagged protein detection Myc tag antibodies are used to visualize proteins labelled with this tag in a variety of applications. What is the molecular weight of Myc tag? Anti-Myc tag [9E10] (ab206486) specifically detects a band for Myc tag (UniProt: P01106) at a molecular weight of 48kDa. Trusted by the scientific community Anti-Myc tag [9E10] (ab206486) was first used in a scientific publication in 2015 and has been cited over 10 times in peer-reviewed journals. Other related products We have a range of other formats of antibody clone [9E10] also available for your convenience: ab32 , Carrier free - ab117511 , Biotin - ab197139 , ab206486, Alexa Fluor® 594 - ab223894 , Alexa Fluor® 647 - ab223895 , APC - ab223896

Host

Rat

Application

WB, IHC-Fr, IHC-P, IP, ICC/IF

Platform ID

BAB830324455

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Myc tag antibody [9E10]
Cat. No.AB206486
HostRat
IsotypeIgG1
ApplicationWB, IHC-Fr, IHC-P, IP, ICC/IF
ClonalityMonoclonal
Clone Number9E10
Concentration1 mg/mL Batch dependent concentration
ImmunogenSynthetic Peptide within Human MYC aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.02% Proclin 300 Constituents: PBS
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808). See full target information MYC

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