Anti-Myoglobin antibody [EP3081Y],Abcam,AB77232

What is this antibody validated in? Anti-Myoglobin antibody [EP3081Y] (ab77232) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of Myoglobin? Anti-Myoglobin [EP3081Y] (ab77232) specifically detects a band for Myoglobin (UniProt: P02144) at a molecular weight of 17kDa. Trusted by the scientific community Anti-Myoglobin [EP3081Y] (ab77232) was first used in a scientific publication in 2009 and has been cited over 30 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [EP3081Y] also available for your convenience: ab77232, HRP - ab199243 , Carrier free - ab232388 , Alexa Fluor® 488 - ab320144 , Alexa Fluor® 647 - ab320145 , Alexa Fluor® 555 - ab320146 , Alexa Fluor® 594 - ab320147 , PE - ab320148 , APC - ab320149 , Alexa Fluor® 750 - ab321732 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

WB, IHC-P, ICC/IF

Platform ID

BAB894304994

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Myoglobin antibody [EP3081Y]
Cat. No.AB77232
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationWB, IHC-P, ICC/IF
ClonalityMonoclonal
Clone NumberEP3081Y
Concentration0.154 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand (PubMed : 30918256, PubMed : 34679218). Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide (PubMed : 32891753). Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity (PubMed : 34679218). See full target information MB

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