Anti-NADH2 antibody [9E12-1B3] - N-terminal,Abcam,AB219821

Western blot advice : Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

Host

Mouse

Reactivity

Human

Application

WB

Platform ID

BAB707760785

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-NADH2 antibody [9E12-1B3] - N-terminal
Cat. No.AB219821
HostMouse
IsotypeIgG2a
ReactivityHuman
ApplicationWB
ClonalityMonoclonal
Clone Number9E12-1B3
Concentration1 mg/mL Batch dependent concentration
ImmunogenSynthetic Peptide within Human MT-ND2. The exact immunogen used to generate this antibody is proprietary information.
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 Preservative: 0.02% Sodium azide Constituents: 0.87% Sodium chloride, 0.36% HEPES
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 16996290). Essential for the catalytic activity and assembly of complex I (PubMed : 16996290). See full target information MT-ND2

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