Anti-NEDD8 antibody [Y297],Abcam,AB81264
What is this antibody validated in? Anti-NEDD8 antibody [Y297] (ab81264) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of NEDD8? Anti-NEDD8 [Y297] (ab81264) specifically detects a band for NEDD8 (UniProt: Q15843) at a molecular weight of 9kDa. Trusted by the scientific community Anti-NEDD8 [Y297] (ab81264) was first used in a scientific publication in 2009 and has been cited over 40 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [Y297] also available for your convenience: ab81264, Alexa Fluor® 647 - ab200849 , Alexa Fluor® 488 - ab201249 , Alexa Fluor® 594 - ab207247 , Alexa Fluor® 405 - ab207248 , Alexa Fluor® 555 - ab207249 , Carrier free - ab220816 , PE - ab305725 , APC - ab305726 , HRP - ab305727 , Alkaline Phosphatase - ab308828 , Alexa Fluor® 568 - ab312568 , Alexa Fluor® 750 - ab321737 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
ICC/IF, WB, IHC-P, IP, Flow Cyt (Intra)
Platform ID
BAB523115664
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53 (PubMed : 10318914, PubMed : 10597293, PubMed : 11953428, PubMed : 14690597, PubMed : 15242646, PubMed : 9694792, PubMed : 38605244, PubMed : 38316879). Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (PubMed : 10318914, PubMed : 10597293, PubMed : 11953428, PubMed : 20688984, PubMed : 9694792, PubMed : 38605244, PubMed : 38316879). Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity (PubMed : 15242646). Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M (PubMed : 14690597). See full target information NEDD8
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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