Anti-NLRP3 antibody [EPR23073-96],Abcam,AB270449
What is this antibody validated in? Anti-NLRP3 antibody [EPR23073-96] (ab270449) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples. What is the molecular weight of NLRP3? Anti-NLRP3 [EPR23073-96] (ab270449) specifically detects a band for NLRP3 (UniProt: Q8R4B8) at a molecular weight of 118kDa. Trusted by the scientific community Anti-NLRP3 [EPR23073-96] (ab270449) was first used in a scientific publication in 2020 and has been cited over 20 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-NLRP3 antibody [EPR23073-96] (ab270449) has been confirmed by Western blot testing in Nlrp3 Knockout Mouse BMDCs. Other related products We have a range of other formats of antibody clone [EPR23073-96] also available for your convenience: ab270449, Carrier free - ab272702 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse
Application
WB, ICC/IF, IP
Platform ID
BAB777638492
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis (PubMed : 19362020, PubMed : 23582325, PubMed : 26642356, PubMed : 26814970, PubMed : 27374331, PubMed : 27929086, PubMed : 28656979, PubMed : 28847925, PubMed : 30518920, PubMed : 36178239). In response to pathogens and other damage-associated signals that affect the integrity of membranes, initiates the formation of the inflammasome polymeric complex composed of NLRP3, CASP1 and PYCARD/ASC (PubMed : 16407889, PubMed : 18403674, PubMed : 19362020, PubMed : 26642356, PubMed : 26814970, PubMed : 27374331, PubMed : 28847925). Recruitment of pro-caspase-1 (proCASP1) to the NLRP3 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion and pyroptosis (PubMed : 16546100, PubMed : 17008311, PubMed : 26642356, PubMed : 26814970, PubMed : 27374331, PubMed : 28847925). Activation of NLRP3 inflammasome is also required for HMGB1 secretion; stimulating inflammatory responses (PubMed : 22801494). Under resting conditions, ADP-bound NLRP3 is autoinhibited (By similarity). NLRP3 activation stimuli include extracellular ATP, nigericin, reactive oxygen species, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, such as asbestos, silica, aluminum salts, cytosolic dsRNA, etc (PubMed : 16407888, PubMed : 16407889, PubMed : 16407890, PubMed : 18403674, PubMed : 19362020, PubMed : 37001519). Almost all stimuli trigger intracellular K(+) efflux (PubMed : 23809161). These stimuli lead to membrane perturbation and activation of NLRP3 (By similarity). Upon activation, NLRP3 is transported to microtubule organizing center (MTOC), where it is unlocked by NEK7, leading to its relocalization to dispersed trans-Golgi network (dTGN) vesicle membranes and formation of an active inflammasome complex (PubMed : 26814970, PubMed : 34615873, PubMed : 34861190). Associates with dTGN vesicle membranes by binding to phosphatidylinositol 4-phosphate (PtdIns4P) (PubMed : 30487600). Shows ATPase activity (PubMed : 34861190).. Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth (PubMed : 26098997). During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription (PubMed : 26098997). Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3' (PubMed : 26098997). May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (PubMed : 26098997). See full target information Nlrp3
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
Please provide the required information below so that we can quickly source your products.