Anti-NQO1 antibody [EPR3309],Abcam,AB80588
What is this antibody validated in? Anti-NQO1 antibody [EPR3309] (ab80588) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of NQO1? Anti-NQO1 [EPR3309] (ab80588) specifically detects a band for NQO1 (UniProt: P15559) at a molecular weight of 31kDa. Trusted by the scientific community Anti-NQO1 [EPR3309] (ab80588) was first used in a scientific publication in 2009 and has been cited over 130 times in peer-reviewed journals. Reviewed by scientists Anti-NQO1 [EPR3309] (ab80588) has over 5 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-NQO1 antibody [EPR3309] (ab80588) has been confirmed by Western blot testing in NQO1 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR3309] also available for your convenience: ab80588, Alexa Fluor® 647 - ab196196 , Alexa Fluor® 488 - ab196465 , Carrier free - ab239896 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
Flow Cyt (Intra), IP, ICC/IF, WB
Platform ID
BAB030062328
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species (By similarity) (PubMed : 8999809, PubMed : 9271353). Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion (PubMed : 15102952, PubMed : 8999809, PubMed : 9271353). Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential (PubMed : 8999809). Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome (PubMed : 15687255, PubMed : 28291250). See full target information NQO1
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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