Anti-Niemann Pick C1 antibody [EPR5209] - BSA and Azide free,Abcam,AB224268
What is this antibody validated in? Anti-Niemann Pick C1 antibody [EPR5209] - BSA and Azide free (ab224268) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of Niemann Pick C1? Anti-Niemann Pick C1 [EPR5209] - BSA and Azide free (ab224268) specifically detects a band for Niemann Pick C1 (UniProt: O15118) at a molecular weight of 142kDa. Specificity confirmed The specificity of Anti-Niemann Pick C1 antibody [EPR5209] - BSA and Azide free (ab224268) has been confirmed by Western blot testing in Niemann Pick C1 Knockout HAP1 cell line. Other related products We have a range of other formats of antibody clone [EPR5209] also available for your convenience: ab134113 , APC - ab223985 , Carrier free - ab224268, PE - ab317903 , Alexa Fluor® 488 - ab317983 , Alexa Fluor® 594 - ab318025 , Alexa Fluor® 555 - ab318067 , Alexa Fluor® 647 - ab318108 , Alexa Fluor® 750 - ab321075 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
IHC-P, Flow Cyt (Intra), WB, ICC/IF
Platform ID
BAB464132725
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment (PubMed : 10821832, PubMed : 12554680, PubMed : 18772377, PubMed : 27238017, PubMed : 9211849, PubMed : 9927649). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1 (PubMed : 18772377, PubMed : 19563754, PubMed : 27238017, PubMed : 27378690, PubMed : 28784760, PubMed : 9211849, PubMed : 9927649). Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket (PubMed : 19563754). Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable). Inhibits cholesterol-mediated mTORC1 activation throught its interaction with SLC38A9 (PubMed : 28336668).. (Microbial infection) Acts as an endosomal entry receptor for ebolavirus. See full target information NPC intracellular cholesterol transporter 1
Category Paths
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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