Anti-Nrf2 antibody,Abcam,AB137550
Anti-Nrf2 antibody (ab137550) is a rabbit polyclonal antibody and is validated for use in ChIP, IP, WB in human, mouse, rat samples. Anti-Nrf2 antibody (ab137550) has been cited over 408 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-Nrf2 antibody (ab137550) has high sensitivity and specificity. The specificity of Anti-Nrf2 antibody (ab137550) has been confirmed by testing in knockout samples. Anti-Nrf2 antibody (ab137550) has 7 independent reviews from customers. Anti-Nrf2 antibody (ab137550) specifically detects Nrf2 (UniProt ID: Q16236; Molecular weight: 68kDa) and is sold in 50 µL selling sizes. NRF2 (Nuclear Factor Erythroid 2-Related Factor 2) is a transcription factor that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. It plays a crucial role in cellular defense mechanisms. Dysregulation of NRF2 has been implicated in various diseases, including neurodegenerative disorders like Alzheimer's (AD) and Parkinson's disease (PD), where oxidative stress and inflammation are key pathological features. NRF2 is bound to ubiquitin when it is the nucleus so MW would be higher than cytoplasm. Please note: Nrf2 antibody (ab137550) detects no signal in most untreated samples for WB. Stimuli treated samples are recommended. Nrf2 expression is stimulated by oxidative stress, electrophiles and chemical activators (PMID: 25761198, PMID: 27638861 and PMID: 28587109). WB protocol info included in legend for MDA-MB-231 nuclear extract treated with 30 μM tBHQ for 4 hours (+). Abcam recommended secondaries - Goat Anti-Rabbit HRP ( ab205718 ) and Goat Anti-Rabbit Alexa Fluor®488 ( ab150077 ). Or search our wide range of secondary antibodies for use with your experiment.
Host
Rabbit
Reactivity
Human
Application
WB, IP, ChIP
Platform ID
BAB842918407
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data The protein expressed by the NFE2L2 gene functions as a transcription factor that plays a key role in responding to oxidative stress by binding to antioxidant response elements (ARE) in the promoter regions of various cytoprotective genes, such as phase 2 detoxifying enzymes, to promote their expression and neutralize reactive electrophiles. Under normal conditions, it is ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex. However, oxidative stress leads to the inhibition of the BCR(KEAP1) complex, allowing NFE2L2/NRF2 to accumulate in the nucleus, form heterodimers with small Maf proteins, and bind to ARE elements. Additionally, the NFE2L2/NRF2 pathway is activated by selective autophagy, where KEAP1 interacts with SQSTM1/p62, inactivating the BCR(KEAP1) complex and leading to nuclear NFE2L2/NRF2 accumulation and cytoprotective gene expression. It may also be involved in activating genes of the beta-globin cluster by facilitating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. This supplementary information is collated from multiple sources and compiled automatically. See full target information NFE2L2
Category Paths
- Products>Primary Antibodies>Polyclonal Antibodies
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