Anti-Nucleolin antibody [364-5],Abcam,AB136649

What is this antibody validated in? Anti-Nucleolin antibody [364-5] (ab136649) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples. What is the molecular weight of Nucleolin? Anti-Nucleolin [364-5] (ab136649) specifically detects a band for Nucleolin (UniProt: P19338) at a molecular weight of 76kDa. Trusted by the scientific community Anti-Nucleolin [364-5] (ab136649) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals. Other related products We have a range of other formats of antibody clone [364-5] also available for your convenience: ab136649, Alexa Fluor® 488 - ab154028 , Alexa Fluor® 647 - ab198580 , Carrier free - ab212672 Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected]

Host

Mouse

Reactivity

Human

Application

WB, ICC/IF, Flow Cyt, IP, IHC-P

Platform ID

BAB093842963

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Nucleolin antibody [364-5]
Cat. No.AB136649
HostMouse
IsotypeIgG1
ReactivityHuman
ApplicationWB, ICC/IF, Flow Cyt, IP, IHC-P
ClonalityMonoclonal
Clone Number364-5
Concentration1 mg/mL Batch dependent concentration
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.88% Sodium chloride, 0.36% HEPES
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. See full target information NCL

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