Anti-Nucleolin antibody [EPR7952],Abcam,AB129200

What is this antibody validated in? Anti-Nucleolin antibody [EPR7952] (ab129200) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of Nucleolin? Anti-Nucleolin [EPR7952] (ab129200) specifically detects a band for Nucleolin (UniProt: P19338) at a molecular weight of 76kDa. Trusted by the scientific community Anti-Nucleolin [EPR7952] (ab129200) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [EPR7952] also available for your convenience: ab129200, Alexa Fluor® 488 - ab202708 , Alexa Fluor® 647 - ab202709 , Alexa Fluor® 555 - ab203417 , Carrier free - ab226113 , PE - ab305946 , APC - ab305947 , HRP - ab305948 , Alkaline Phosphatase - ab308818 , Alexa Fluor® 594 - ab310551 , Alexa Fluor® 568 - ab312558 , Alexa Fluor® 750 - ab321037 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

ICC/IF, Flow Cyt (Intra), WB, IHC-P

Platform ID

BAB281386692

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Nucleolin antibody [EPR7952]
Cat. No.AB129200
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationICC/IF, Flow Cyt (Intra), WB, IHC-P
ClonalityMonoclonal
Clone NumberEPR7952
Concentration0.13 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. See full target information NCL

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