Anti-Oct4 antibody [GT486],Abcam,AB184665

What is this antibody validated in? Anti-Oct4 antibody [GT486] (ab184665) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples. What is the molecular weight of Oct4? Anti-Oct4 [GT486] (ab184665) specifically detects a band for Oct4 (UniProt: Q01860) at a molecular weight of 39kDa. Trusted by the scientific community Anti-Oct4 [GT486] (ab184665) was first used in a scientific publication in 2014 and has been cited over 30 times in peer-reviewed journals.

Host

Mouse

Reactivity

Mouse, Human

Application

Flow Cyt, IP, ICC/IF, WB, IHC-P

Platform ID

BAB408148346

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-Oct4 antibody [GT486]
Cat. No.AB184665
HostMouse
IsotypeIgG2b
ReactivityMouse, Human
ApplicationFlow Cyt, IP, ICC/IF, WB, IHC-P
ClonalityMonoclonal
Clone NumberGT486
Concentration1 mg/mL Batch dependent concentration
ImmunogenRecombinant Fragment Protein within Human POU5F1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein G
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. See full target information POU5F1

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