Anti-PADI4 / PAD4 antibody,Abcam,AB50247

We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 18th July 2017. The following lots is/are still unpurified and still in stock as of 18th July 2017 - GR316454-5, GR316454-6, GR316454-7, GR316454-8, GR316454-9, GR316454-10. Lot numbers other than GR316454-5, GR316454-6, GR316454-7, GR316454-8, GR316454-9, GR316454-10 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

Host

Rabbit

Reactivity

Human

Application

ELISA, IP, ICC/IF, WB

Platform ID

BAB975577229

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-PADI4 / PAD4 antibody
Cat. No.AB50247
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationELISA, IP, ICC/IF, WB
ClonalityPolyclonal
Concentration0.5 mg/mL Batch dependent concentration
ImmunogenSynthetic Peptide within Human PADI4. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationConstituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1 : citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed : 18209087). Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (PubMed : 15345777). Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (PubMed : 15731352). See full target information PADI4

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