Anti-PADI4 / PAD4 antibody [OTI4H5],Abcam,AB128086

What is this antibody validated in? Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Human samples. What is the molecular weight of PADI4 / PAD4? Anti-PADI4 / PAD4 [OTI4H5] (ab128086) specifically detects a band for PADI4 / PAD4 (UniProt: Q9UM07) at a molecular weight of 74kDa. Trusted by the scientific community Anti-PADI4 / PAD4 [OTI4H5] (ab128086) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.

Host

Mouse

Reactivity

Human, African green monkey

Application

Flow Cyt (Intra), IHC-P, WB, ICC/IF

Platform ID

BAB475627205

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-PADI4 / PAD4 antibody [OTI4H5]
Cat. No.AB128086
HostMouse
IsotypeIgG2a
ReactivityHuman, African green monkey
ApplicationFlow Cyt (Intra), IHC-P, WB, ICC/IF
ClonalityMonoclonal
Clone NumberOTI4H5
Concentration1 mg/mL Batch dependent concentration
ImmunogenRecombinant Full Length Protein corresponding to Human PADI4.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1 : citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed : 18209087). Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (PubMed : 15345777). Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (PubMed : 15731352). See full target information PADI4

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