Anti-PARK7/DJ1 antibody [MJF-R16 (66-5)] - BSA and Azide free,Abcam,AB218374
ab218374 is the carrier-free version of ab169520 . In recent years, a critical need in the Parkinson's Disease (PD) research community has been access to well-characterized antibodies directed against known PD-relevant proteins. The Michael J. Fox Foundation (MJFF) has supported this effort by partnering with Drs. Un Kang and David White (University of Chicago) to help accelerate PD research. DJ-1 is widely expressed in the adult mammal and highly conserved between species. Loss-of-function mutations in DJ-1 were recently identified in an autosomal recessive form of Parkinson's disease (PARK7). Among other roles, DJ-1 protects cells against oxidative stress. Oxidization of the cysteine 106 residue (C106) of DJ-1 occurs as a consequence oxidative stress, but is also necessary to fully activate DJ-1 functions. The oxidation state of DJ-1 C106 appears to lead to distinct roles for DJ-1 in the cellular response to oxidative stress. Oxidation of C106 to the sulfinic (-SO2H) form has been implicated as a necessary step to achieve optimal protective functions of DJ-1, whereas oxidation to the sulfonic form (-SO3H) results in the oxidative destabilization of DJ-1 structure. The sulfonic form has been identified as a major oxidized form in PD brains. With the generation of this critical research tool, MJFF hopes to ensure that the role of this modification can be further investigated by all researchers and the relevance of oxidized forms of DJ-1 can be more definitively examined in Parkinson's disease. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Species reactivity Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc. Collaborations This antibody was developed with support from The Michael J. Fox Foundation.
Host
Rabbit
Reactivity
Human
Application
WB
Platform ID
BAB852613751
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Multifunctional protein with controversial molecular function which plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease (PubMed : 12796482, PubMed : 17015834, PubMed : 18711745, PubMed : 19229105, PubMed : 20304780, PubMed : 25416785, PubMed : 26995087, PubMed : 28993701). It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway (PubMed : 12612053, PubMed : 14749723, PubMed : 15502874, PubMed : 17015834, PubMed : 18711745, PubMed : 21097510). Has been described as a protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals (PubMed : 25416785, PubMed : 28596309). But this function is rebuted by other works (PubMed : 27903648, PubMed : 31653696). As a protein deglycase, repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage (PubMed : 25416785, PubMed : 26995087, PubMed : 28013050). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair (PubMed : 28596309). Protects histones from adduction by methylglyoxal, controls the levels of methylglyoxal-derived argininine modifications on chromatin (PubMed : 30150385). Able to remove the glycations and restore histone 3, histone glycation disrupts both local and global chromatin architecture by altering histone-DNA interactions as well as histone acetylation and ubiquitination levels (PubMed : 30150385, PubMed : 30894531). Displays a very low glyoxalase activity that may reflect its deglycase activity (PubMed : 22523093, PubMed : 28993701, PubMed : 31653696). Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death (PubMed : 16390825). Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria (PubMed : 16632486, PubMed : 19229105). Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking (PubMed : 18711745). Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells (PubMed : 23847046). In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner. Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting (By similarity). Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress (PubMed : 18626009). Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity (PubMed : 23792957). In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis (By similarity). See full target information PARK7
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