Anti-PDHA1 antibody [EPR11098],Abcam,AB168379

What is this antibody validated in? Anti-PDHA1 antibody [EPR11098] (ab168379) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of PDHA1? Anti-PDHA1 [EPR11098] (ab168379) specifically detects a band for PDHA1 (UniProt: P08559) at a molecular weight of 43kDa. Trusted by the scientific community Anti-PDHA1 [EPR11098] (ab168379) was first used in a scientific publication in 2013 and has been cited over 30 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-PDHA1 antibody [EPR11098] (ab168379) has been confirmed by Western blot testing in PDHA1 Knockout HeLa cells. Other related products We have a range of other formats of antibody clone [EPR11098] also available for your convenience: ab168379, Carrier free - ab176835 , PE - ab305779 , HRP - ab305781 , Alkaline Phosphatase - ab308723 , Alexa Fluor® 488 - ab309686 , Alexa Fluor® 647 - ab310050 , Alexa Fluor® 594 - ab310440 , Alexa Fluor® 555 - ab311968 , Alexa Fluor® 750 - ab321159 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)

Platform ID

BAB418943162

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-PDHA1 antibody [EPR11098]
Cat. No.AB168379
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationWB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
ClonalityMonoclonal
Clone NumberEPR11098
Concentration0.363 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Together with PDHB forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex (PubMed : 17474719, PubMed : 19081061). The PDH complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (PubMed : 19081061, PubMed : 7782287). It contains multiple copies of three enzymatic components : pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Probable). The E1 subunit catalyzes both the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of a lipoyl group covalently linked to the lipoyl-bearing domains of E2 (PubMed : 17474719, PubMed : 19081061, PubMed : 7782287). See full target information PDHA1

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