Anti-PERK antibody [EPR19876-294],Abcam,AB229912
What is this antibody validated in? Anti-PERK antibody [EPR19876-294] (ab229912) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples. What is the molecular weight of PERK? Anti-PERK [EPR19876-294] (ab229912) specifically detects a band for PERK (UniProt: Q9NZJ5) at a molecular weight of 125kDa. Trusted by the scientific community Anti-PERK [EPR19876-294] (ab229912) was first used in a scientific publication in 2019 and has been cited over 40 times in peer-reviewed journals. Reviewed by scientists Anti-PERK [EPR19876-294] (ab229912) has over 5 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-PERK antibody [EPR19876-294] (ab229912) has been confirmed by Western blot testing in EIF2AK3 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR19876-294] also available for your convenience: ab229912, Carrier free - ab254249 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
WB
Platform ID
BAB807923948
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress, such as unfolded protein response (UPR) (PubMed : 10026192, PubMed : 10677345, PubMed : 11907036, PubMed : 12086964, PubMed : 25925385, PubMed : 31023583). Key effector of the integrated stress response (ISR) to unfolded proteins : EIF2AK3/PERK specifically recognizes and binds misfolded proteins, leading to its activation and EIF2S1/eIF-2-alpha phosphorylation (PubMed : 10677345, PubMed : 27917829, PubMed : 31023583). EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed : 10026192, PubMed : 10677345, PubMed : 31023583, PubMed : 33384352). The EIF2AK3/PERK-mediated unfolded protein response increases mitochondrial oxidative phosphorylation by promoting ATF4-mediated expression of COX7A2L/SCAF1, thereby increasing formation of respiratory chain supercomplexes (PubMed : 31023583). In contrast to most subcellular compartments, mitochondria are protected from the EIF2AK3/PERK-mediated unfolded protein response due to EIF2AK3/PERK inhibition by ATAD3A at mitochondria-endoplasmic reticulum contact sites (PubMed : 39116259). In addition to EIF2S1/eIF-2-alpha, also phosphorylates NFE2L2/NRF2 in response to stress, promoting release of NFE2L2/NRF2 from the BCR(KEAP1) complex, leading to nuclear accumulation and activation of NFE2L2/NRF2 (By similarity). Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) (By similarity). Involved in control of mitochondrial morphology and function (By similarity). See full target information EIF2AK3
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