Anti-PHD finger protein 1/PHF1 antibody [EPR14222],Abcam,AB184951

Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Rat, Human, Mouse

Application

Flow Cyt (Intra), IHC-P, WB, ICC/IF, ChIC/CUT&RUN-seq, IP

Platform ID

BAB809423228

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-PHD finger protein 1/PHF1 antibody [EPR14222]
Cat. No.AB184951
HostRabbit
IsotypeIgG
ReactivityRat, Human, Mouse
ApplicationFlow Cyt (Intra), IHC-P, WB, ICC/IF, ChIC/CUT&RUN-seq, IP
ClonalityMonoclonal
Clone NumberEPR14222
Concentration0.796 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex : it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed : 18285464, PubMed : 23273982). According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed : 23142980). Regulates p53/TP53 stability and prolonges its turnover : may act by specifically binding to a methylated from of p53/TP53. See full target information PHF1

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.