Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y],Abcam,AB40776
What is this antibody validated in? Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (ab40776) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of PI 3 Kinase catalytic subunit alpha/PIK3CA? Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA [EP383Y] (ab40776) specifically detects a band for PI 3 Kinase catalytic subunit alpha/PIK3CA (UniProt: P42336) at a molecular weight of 110kDa. Trusted by the scientific community Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA [EP383Y] (ab40776) was first used in a scientific publication in 2006 and has been cited over 80 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (ab40776) has been confirmed by Western blot testing in PIK3CA Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EP383Y] also available for your convenience: ab40776, Carrier free - ab185927 , Alexa Fluor® 488 - ab202671 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
Flow Cyt (Intra), ICC/IF, WB, IP
Platform ID
BAB998066429
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 15135396, PubMed : 23936502, PubMed : 28676499). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396, PubMed : 28676499). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed : 23936502, PubMed : 28676499). Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity). See full target information PIK3CA
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