Anti-PRAME antibody [EPR20330],Abcam,AB219650

Anti-PRAME antibody [EPR20330] (ab219650) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB. Abcam's high quality manufacturing and validation processes ensure Anti-PRAME antibody [EPR20330] (ab219650) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-PRAME antibody [EPR20330] (ab219650) specifically detects PRAME (UniProt ID: P78395; Molecular weight: 58kDa) and is sold in 100 µL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR20330 - ab232571 . Antibody clone EPR20330 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 555, Alexa Fluor ® 647, Alexa Fluor ® 488, Alexa Fluor ® 594 ( ab307626 , ab307630 , ab307769 , ab312896 ). This top cited antibody is highly specific for PRAME, a protein that is highly expressed in different types of cancers and is involved in cell proliferation and metastasis, as well as the outcomes of patients with cancer. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Human

Application

Flow Cyt (Intra), WB, IHC-P, IP, ICC/IF

Platform ID

BAB692656774

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-PRAME antibody [EPR20330]
Cat. No.AB219650
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationFlow Cyt (Intra), WB, IHC-P, IP, ICC/IF
ClonalityMonoclonal
Clone NumberEPR20330
Concentration0.51 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation (PubMed : 21822215, PubMed : 26138980). The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding (PubMed : 26138980). In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation (PubMed : 21822215). Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG : prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis (PubMed : 16179254). See full target information PRAME

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