Anti-Perforin antibody [CB5.4],Abcam,AB16074

What is this antibody validated in? Anti-Perforin antibody [CB5.4] (ab16074) is a rat monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples. Trusted by the scientific community Anti-Perforin [CB5.4] (ab16074) was first used in a scientific publication in 2004 and has been cited over 30 times in peer-reviewed journals. Reviewed by scientists Anti-Perforin [CB5.4] (ab16074) has over 5 independent reviews from customers.

Host

Rat

Reactivity

Mouse

Application

WB, ICC/IF, IHC-Fr, IP, IHC-P, ICC

Platform ID

BAB807424188

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-Perforin antibody [CB5.4]
Cat. No.AB16074
HostRat
IsotypeIgG2a
ReactivityMouse
ApplicationWB, ICC/IF, IHC-Fr, IP, IHC-P, ICC
ClonalityMonoclonal
Clone NumberCB5.4
ImmunogenRecombinant Fragment Protein within Mouse Prf1 aa 50-550. The exact immunogen used to generate this antibody is proprietary information.
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.02% Sodium azide Constituents: PBS
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed : 19446473, PubMed : 21037563, PubMed : 26306037, PubMed : 2783478, PubMed : 3261391, PubMed : 35148176, PubMed : 35705808, PubMed : 7520535, PubMed : 7526382, PubMed : 7972104, PubMed : 8164737). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed : 19446473, PubMed : 21037563, PubMed : 26306037, PubMed : 3261391, PubMed : 35148176, PubMed : 35705808, PubMed : 7526382, PubMed : 8164737). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed : 19446473, PubMed : 21037563, PubMed : 26306037, PubMed : 3261391, PubMed : 35148176, PubMed : 7526382, PubMed : 8164737). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (By similarity). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (PubMed : 35705808). See full target information Prf1

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