Anti-RBM20 antibody,Abcam,AB233147

What is this antibody validated in? Anti-RBM20 antibody (ab233147) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Pig samples. What is the molecular weight of RBM20? Anti-RBM20 (ab233147) specifically detects a band for RBM20 (UniProt: Q5T481) at a molecular weight of 134kDa.

Host

Rabbit

Reactivity

Pig, Human

Application

IHC-P, WB

Platform ID

BAB578688976

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-RBM20 antibody
Cat. No.AB233147
HostRabbit
IsotypeIgG
ReactivityPig, Human
ApplicationIHC-P, WB
ClonalityPolyclonal
Concentration0.5 mg/mL Batch dependent concentration
ImmunogenRecombinant Fragment Protein within Human RBM20 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes encoding key structural proteins involved in cardiac development, such as TTN (Titin), CACNA1C, CAMK2D or PDLIM5/ENH (PubMed : 22466703, PubMed : 24960161, PubMed : 26604136, PubMed : 27496873, PubMed : 27531932, PubMed : 29895960, PubMed : 30948719, PubMed : 32840935, PubMed : 34732726, PubMed : 35427468). Acts as a repressor of mRNA splicing : specifically binds the 5'UCUU-3' motif that is predominantly found within intronic sequences of pre-mRNAs, leading to the exclusion of specific exons in target transcripts (PubMed : 24960161, PubMed : 30948719, PubMed : 34732726). RBM20-mediated exon skipping is hormone-dependent and is essential for TTN isoform transition in both cardiac and skeletal muscles (PubMed : 27531932, PubMed : 30948719). RBM20-mediated exon skipping of TTN provides substrates for the formation of circular RNA (circRNAs) from the TTN transcripts (PubMed : 27531932, PubMed : 34732726). Together with RBM24, promotes the expression of short isoforms of PDLIM5/ENH in cardiomyocytes (By similarity). See full target information RBM20

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