Anti-RIG-I/DDX58 antibody [EPR18629] - BSA and Azide free,Abcam,AB240230
ab240230 is the carrier-free version of ab180675 . Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.
Host
Rabbit
Reactivity
Human
Application
WB, IP
Platform ID
BAB582287248
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Innate immune receptor that senses cytoplasmic viral nucleic acids and activates a downstream signaling cascade leading to the production of type I interferons and pro-inflammatory cytokines (PubMed : 15208624, PubMed : 15708988, PubMed : 16125763, PubMed : 16127453, PubMed : 16153868, PubMed : 17190814, PubMed : 18636086, PubMed : 19122199, PubMed : 19211564, PubMed : 24366338, PubMed : 28469175, PubMed : 29117565, PubMed : 31006531, PubMed : 34935440, PubMed : 35263596, PubMed : 36793726). Forms a ribonucleoprotein complex with viral RNAs on which it homooligomerizes to form filaments (PubMed : 15208624, PubMed : 15708988). The homooligomerization allows the recruitment of RNF135 an E3 ubiquitin-protein ligase that activates and amplifies the RIG-I-mediated antiviral signaling in an RNA length-dependent manner through ubiquitination-dependent and -independent mechanisms (PubMed : 28469175, PubMed : 31006531). Upon activation, associates with mitochondria antiviral signaling protein (MAVS/IPS1) that activates the IKK-related kinases TBK1 and IKBKE which in turn phosphorylate the interferon regulatory factors IRF3 and IRF7, activating transcription of antiviral immunological genes including the IFN-alpha and IFN-beta interferons (PubMed : 28469175, PubMed : 31006531). Ligands include 5'-triphosphorylated ssRNAs and dsRNAs but also short dsRNAs (<1 kb in length) (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae : Human respiratory syncytial virus and measles virus (MeV), Rhabdoviridae : vesicular stomatitis virus (VSV), Orthomyxoviridae : influenza A and B virus, Flaviviridae : Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV) (PubMed : 21616437, PubMed : 21884169). It also detects rotaviruses and reoviruses (PubMed : 21616437, PubMed : 21884169). Detects and binds to SARS-CoV-2 RNAs which is inhibited by m6A RNA modifications (Ref.71). Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV) (PubMed : 19631370). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration. See full target information RIGI
Category Paths
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