Anti-RPA32/RPA2 antibody [EPR2877Y],Abcam,AB76420
What is this antibody validated in? Anti-RPA32/RPA2 antibody [EPR2877Y] (ab76420) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of RPA32/RPA2? Anti-RPA32/RPA2 [EPR2877Y] (ab76420) specifically detects a band for RPA32/RPA2 (UniProt: P15927) at a molecular weight of 29kDa. Trusted by the scientific community Anti-RPA32/RPA2 [EPR2877Y] (ab76420) was first used in a scientific publication in 2009 and has been cited over 20 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [EPR2877Y] also available for your convenience: ab76420, Alexa Fluor® 647 - ab225034 , Alexa Fluor® 488 - ab225035 , Carrier free - ab236044 , PE - ab305805 , APC - ab305806 , HRP - ab305807 , Alkaline Phosphatase - ab308696 , Alexa Fluor® 594 - ab310406 , Alexa Fluor® 555 - ab311933 , Alexa Fluor® 568 - ab312404 , Alexa Fluor® 750 - ab321727 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
IHC-P, ICC/IF, Flow Cyt (Intra), IP, WB
Platform ID
BAB147117379
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed : 17596542). See full target information RPA2
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