Anti-RUNX2 antibody [EPR14334],Abcam,AB192256

Product Specifications Anti-RUNX2 antibody [EPR14334] (ab192256) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, Flow Cyt (Intra), ICC/IF, IHC-P and WB in human, mouse and rat samples. Anti-RUNX2 antibody [EPR14334] (ab192256) specifically detects RUNX2 (UniProt ID: Q13950; Molecular weight: 57kDa) and is sold in 100 µL and 1 mL selling sizes. Quality and Validation Abcam's high quality manufacturing and validation processes ensure Anti-RUNX2 antibody [EPR14334] (ab192256) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-RUNX2 antibody [EPR14334] (ab192256) has been cited over 114 times in peer reviewed journals and is trusted by the scientific community. Anti-RUNX2 antibody [EPR14334] (ab192256) has 8 independent reviews from customers. Related Products Antibody clone EPR14334 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 ( ab215954 , ab215955 ). Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

WB, Flow Cyt (Intra), IHC-P, ICC/IF, ChIC/CUT&RUN-seq

Platform ID

BAB024453757

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-RUNX2 antibody [EPR14334]
Cat. No.AB192256
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationWB, Flow Cyt (Intra), IHC-P, ICC/IF, ChIC/CUT&RUN-seq
ClonalityMonoclonal
Clone NumberEPR14334
Concentration0.518 - 0.537 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis (PubMed : 28505335, PubMed : 28703881, PubMed : 28738062). Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation : synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation. See full target information RUNX2

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