Anti-Rad51 antibody,Abcam,AB176458

What is this antibody validated in? Anti-Rad51 antibody (ab176458) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Chinese hamster, Human, Mouse, Xenopus laevis samples. What is the molecular weight of Rad51? Anti-Rad51 (ab176458) specifically detects a band for Rad51 (UniProt: Q06609) at a molecular weight of 36kDa. Trusted by the scientific community Anti-Rad51 (ab176458) was first used in a scientific publication in 2013 and has been cited over 20 times in peer-reviewed journals.

Host

Rabbit

Reactivity

Mouse, Human, Xenopus laevis, Chinese hamster

Application

IP, WB, IHC-P, ICC/IF

Platform ID

BAB138821363

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-Rad51 antibody
Cat. No.AB176458
HostRabbit
IsotypeIgG
ReactivityMouse, Human, Xenopus laevis, Chinese hamster
ApplicationIP, WB, IHC-P, ICC/IF
ClonalityPolyclonal
Concentration1 mg/mL Batch dependent concentration
ImmunogenRecombinant Full Length Protein corresponding to Human RAD51.
PurityAffinity purification Immunogen
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data The protein expressed by the RAD51 gene plays an important role in homologous strand exchange, a critical step in DNA repair through homologous recombination (HR). It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity, catalyzing the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. RAD51 binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for the homology search and strand exchange. It is part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C, which is thought to play a role in DNA repair by HR. RAD51 also plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3 and is involved in interstrand cross-link repair. This supplementary information is collated from multiple sources and compiled automatically. See full target information RAD51

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