Anti-Rhodopsin antibody [Rho 4D2],Abcam,AB98887

What is this antibody validated in? Anti-Rhodopsin antibody [Rho 4D2] (ab98887) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Cow, Human, Mouse, Pig, Rat, Xenopus laevis samples. What is the molecular weight of Rhodopsin? Anti-Rhodopsin [Rho 4D2] (ab98887) specifically detects a band for Rhodopsin (UniProt: P02699) at a molecular weight of 38kDa. Trusted by the scientific community Anti-Rhodopsin [Rho 4D2] (ab98887) was first used in a scientific publication in 2010 and has been cited over 50 times in peer-reviewed journals.

Host

Mouse

Reactivity

Mouse, Cow, Human, Pig, Rat, Xenopus laevis

Application

IP, IHC-P, IHC-Fr, ICC, ELISA, Flow Cyt, WB

Platform ID

BAB206899934

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Rhodopsin antibody [Rho 4D2]
Cat. No.AB98887
HostMouse
IsotypeIgG1
ReactivityMouse, Cow, Human, Pig, Rat, Xenopus laevis
ApplicationIP, IHC-P, IHC-Fr, ICC, ELISA, Flow Cyt, WB
ClonalityMonoclonal
Clone NumberRho 4D2
Concentration1 mg/mL Batch dependent concentration
ImmunogenRecombinant Fragment Protein within Cow Rhodopsin. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein G
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Photoreceptor required for image-forming vision at low light intensity (PubMed : 7846071, PubMed : 8107847). Required for photoreceptor cell viability after birth (PubMed : 12566452, PubMed : 2215617). Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed : 26200343, PubMed : 28524165, PubMed : 28753425, PubMed : 8107847). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed : 26200343, PubMed : 28524165). See full target information RHO

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