Anti-SAMHD1 antibody [EPR13040(B)] - BSA and Azide free,Abcam,AB240199
ab240199 is the carrier-free version of ab177462 . Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.
Host
Rabbit
Reactivity
Human
Application
WB, ICC/IF, Flow Cyt (Intra), IHC-P
Platform ID
BAB588784128
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks (PubMed : 19525956, PubMed : 21613998, PubMed : 21720370, PubMed : 22056990, PubMed : 23601106, PubMed : 23602554, PubMed : 24336198, PubMed : 26294762, PubMed : 26431200, PubMed : 28229507, PubMed : 28834754, PubMed : 29670289). Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses, such as HIV-1 : dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells (PubMed : 19525956, PubMed : 21613998, PubMed : 21720370, PubMed : 22056990, PubMed : 23364794, PubMed : 23601106, PubMed : 23602554, PubMed : 24336198, PubMed : 25038827, PubMed : 26101257, PubMed : 26294762, PubMed : 26431200, PubMed : 28229507). Likewise, suppresses LINE-1 retrotransposon activity (PubMed : 24035396, PubMed : 24217394, PubMed : 29610582). Not able to restrict infection by HIV-2 virus; because restriction activity is counteracted by HIV-2 viral protein Vpx (PubMed : 21613998, PubMed : 21720370). In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools (PubMed : 23858451). Phosphorylation at Thr-592 acts as a switch to control dNTPase-dependent and -independent functions : it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks (PubMed : 23601106, PubMed : 23602554, PubMed : 29610582, PubMed : 29670289). Functions during S phase at stalled DNA replication forks to promote the resection of gapped or reversed forks : acts by stimulating the exonuclease activity of MRE11, activating the ATR-CHK1 pathway and allowing the forks to restart replication (PubMed : 29670289). Its ability to promote degradation of nascent DNA at stalled replication forks is required to prevent induction of type I interferons, thereby preventing chronic inflammation (PubMed : 27477283, PubMed : 29670289). Ability to promote DNA end resection at stalled replication forks is independent of dNTPase activity (PubMed : 29670289). Enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation (By similarity). See full target information SAMHD1
Category Paths
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
Please provide the required information below so that we can quickly source your products.