Anti-SDHB antibody [EPR10880],Abcam,AB175225

What is this antibody validated in? Anti-SDHB antibody [EPR10880] (ab175225) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples. What is the molecular weight of SDHB? Anti-SDHB [EPR10880] (ab175225) specifically detects a band for SDHB (UniProt: P21912) at a molecular weight of 32kDa. Trusted by the scientific community Anti-SDHB [EPR10880] (ab175225) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-SDHB antibody [EPR10880] (ab175225) has been confirmed by Western blot testing in SDHB Knockout HEK-293 cells. Other related products We have a range of other formats of antibody clone [EPR10880] also available for your convenience: ab175225, HRP - ab198329 , Biotin - ab198330 , Carrier free - ab249876 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

WB, Flow Cyt (Intra), IP, IHC-P

Platform ID

BAB966670242

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-SDHB antibody [EPR10880]
Cat. No.AB175225
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationWB, Flow Cyt (Intra), IP, IHC-P
ClonalityMonoclonal
Clone NumberEPR10880
Concentration1.027 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed : 26925370, PubMed : 27604842). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). See full target information SDHB

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